The healing ability of left and right colonic anastomoses in rats
10.3760/cma.j.issn.1007-631X.2009.09.018
- VernacularTitle:大鼠左半结肠与右半结肠吻合愈合能力的对比研究
- Author:
Chunhui PENG
;
Yajun CHEN
- Publication Type:Journal Article
- Keywords:
Gastroenterostomy;
Metalloproteinases;
Collagen type Ⅰ;
Rats;
wistar
- From:
Chinese Journal of General Surgery
2009;24(9):740-743
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the healing ability differences between left colonic anastomosis and right colonic anastomosis in rats, in order to find a theoretical basis for the surgical management of colon resection. Methods Forty male Wistar rats were randomly divided into two groups: group A (end-to-end anastomosis of right colon, n=20), and group B (end-to-end anastomosis of left colon, n=20). Half of the rats in each group were sacrificed on the fourth day after operation, and the rest on the seventh day. The expressions of matrix metalloproteinase-1 (MMP-1), MMP-13 and collagen type Ⅰ were shown by immunohistochemical staining, and the histopathologic changes at the anastomosis were also demonstrated. Results The expression of MMP-1, MMP-13 at left colonic anastomosis was higher than that of MMP-1, MMP-13 at right colonic anastomosis, the expression of collagen type Ⅰ was lower than that of collagen type Ⅰ at right colonic anastomosis on the fourth day (P<0.05); The expression of MMP-1 at left colonic anastomosis was higher than that of MMP-1 at right colonic anastomosis, the expression of collagen type Ⅰ was lower than that of collagen type Ⅰ at right colonic anastomosis on the seventh day (P<0.05). Conclusions The healing ability of right colonic anastomosis is higher than that of left colonic anastomosis.
