Deferoxamine pretreatment for hepatic ischemia reperfusion injury in liver auto-transplantation in rats
10.3760/cma.j.issn.1007-631X.2009.09.019
- VernacularTitle:去铁胺对自体肝移植大鼠肝脏缺血再灌注损伤的保护作用
- Author:
Ke LI
;
Wanping CHEN
;
Huanzhou XUE
;
Qingfeng JIANG
;
Qifa YE
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Reperfusion injury;
Deferoxamine;
Cell cytokine;
Oxyradical
- From:
Chinese Journal of General Surgery
2009;24(9):744-747
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of deferoxamine pretreatment for hepatic ischemia reperfusion injury in liver auto-transplantation in rats. Method Murine liver auto-transplantation model was established. Ninety six male Sprague-Dawley rats were randomly divided into three groups: 32 rats in deferoxamine pretreatment group (D), 32 rats in control group with aqua pro injection pretreatment(C) and 32 rats in sham-operation group (S). The animals were killed at 30 min, 2 h, 6 h, 24 h after operation respectively. ALT and AST level, superoxide dismutase (SOD) and malondialdehyde (MDA), liver histological change(HE), the protein expression of HIF-1α、TNF-α and IL-1 were measured. Results At 30 min, 2 h, 6 h, 24 h after operation, the levels of ALT,AST,MDA and the expression of IL-1 protein and TNF-α protein were higher in group C than group D significantly,while the expression of HIF-1α and SOD were higher in group D [SOD(411±70; 384±53; 379±46)、H1F-1α(0.0413±0.0040; 0.0684± 0.0032; 0.0583±0.0032; 0.0491±0.0026)] than group C significantly (P<0.01) [SOD(341±21; 323±25; 303±25)、HIF-1α (0.0254±0.0024; 0.0312±0.0022; 0.0381±0.0022; 0.0257± 0.0015)] (F>59.881;P<0.01). Conclusion The up-regulated expression of HIF-1α, decreased liver lipid peroxidation injury and TNF-α and IL-1 levels, may be involved in the mechanism hy which deferoxamine pretreatment protects liver from ischemia reperfusion injury in rats' liver auto-transplantation.
