Establishment of hyperinsulinemic euglycemic clamp technique and the reference value of insulin sensitivity index in Chinese
10.3760/cma.j.issn.1674-635X.2009.01.006
- VernacularTitle:正常血糖葡萄糖钳夹平台及中国人胰岛素敏感性正常参考值的建立
- Author:
Weigang ZHAO
;
Tao YUAN
;
Qi SUN
;
Yong FU
;
Guohua YANG
;
Yingyue DONG
;
Yaxiu DONG
;
Heng WANG
- Publication Type:Journal Article
- Keywords:
Glucose clamp;
Insulin resistence;
Normal value
- From:
Chinese Journal of Clinical Nutrition
2009;17(1):17-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the technique of hyperinsulinemic euglycemic clamp and to study the reference value of insulin sensitivity index in healthy Chinese. Methods According to the feedback mathematical model developed by DeFronzo, the technique of hyperinsulinemic euglycemic clamp was used in 90 healthy Chi- nese [ male:female =71 = 19; age; (28. 3±6. 1) years; body mass index (20. 9±1.5) kg/m2 ] to study die glu-cose metabolized rate. Blood samples were obtained at timed intervals in the fasting state and during the clamp for the measurement of glucose, insulin and C peptide. Results During the clamp tests, the blood glucose levels were con-trolled within 10% of target value. The coefficient of variation of glucose levels was 3. 8% 0.1%. In the steady state, the insulin sensitivity index (glucose metabolized rate, M value ) was (7.78±2.30) mg· kg-1 min-1, which was distributed normally. The lowest quartile of M value was 6. 286 mg·kg -1 min-1'. The coefficient of variation of M value was 9.4%±2.8%. Conclusion The technique of hyperinsulinemic euglycemic clamp and the reference value of insulin sensitivity index in healthy Chinese are successfully established in our center.
