The study on human osteoclast-like cell formation induced by IL-23-stimulated synovial fibroblasts in rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2009.09.013
- VernacularTitle:白细胞介素-23刺激的类风湿关节炎成纤维细胞对人破骨样细胞形成的研究
- Author:
Xia LI
;
Ru LI
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Interleukins;
Osteoclasts
- From:
Chinese Journal of Rheumatology
2009;13(9):630-632
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of IL-23-stimulated synovial fibroblasts (FLS) in rheumatoid arthritis (RA) on the formation of human osteoclast-like cell. Methods Fibroblasts from patients with RA and osteoarthritis (OA) were stimulated with various concentrations of IL-23 (1, 5 and 10 ng/ml). After incubation for 72 hours, the level of receptor activator of nuclear factor kappa B ligand (RANKL) mRNA were, measured by using real time PCR. Human monocytes (MN) were isolated and cocultured with IL-23-stimulated RA or OA FLS, and subsequently stained for tartrate-resistant acid phosphatase (TRAP) activity. Osteoclast-like cell molecule markers were measured using real time PCR. Results Various concentrations of IL-23 upregulated RANKL expression in rheumatoid FLS, but failed in OA FLS. In the system of IL-23-stimulated RA FLS cocuhured with human MN, TRAP-positive osteoclast-like cell was enhanced and high level expressions of molecule markers were induced. Whereas in the system of non IL-23-stimulated RA FLS or IL-23-stimulated OA FLS cocuhured with human MN there was no TRAP-positive osteoclast-like cell formation. Conclusion The results suggest that IL-23 induced osteoclast-like cell formation is via RANKL expressions in rheumatoid FLS.
