The effect of p53 expression in fibrablast-like synaviocytes on CD4+ T lymphocytes in patients with rheumatoid arthritis

VernacularTitle:成纤维滑膜细胞p53基因表达对类风湿关节炎患者CD4+T淋巴细胞的调节
Author: Bixia TANG ; Xin YOU ; Lidan ZHAO ; Ting ZHANG ; Lei ZHANG ; Xuan ZHANG ; Fulin TANG
Publication Type:Journal Article
Keywords: Arthritis,rheumatoid; Genes,p53; RNA,Small interfering; CD4-positive T-lymp-hocvtes
From: Chinese Journal of Rheumatology 2009;13(9):587-591
CountryChina
Language:Chinese
Abstract: Objective To investigate that p53 expression in fibroblast-like synoviocytes (FLS) and its effects on CD4+ T lymphocytes from active rheumatoid arthritis (RA) patients. Methods Human FLS was transfected with p53siRNA and cocultured with CD4+ T lymphocytes from active RA patients. The expression of osteoprotegerin and IL-6 secretion was detected in the transfected FLS. In addition, the expressions of IFN-γ, IL-17, IL-4 and CD25 as well as mRNA levels of IFN-γ RORγt, IL-17 and Foxp3 in cocuhured CD4+ T lymphocytes were also measured. Results IL-6 secretion decreased in p53-inhibited FLS while osteopro-tegerin expression was not altered, p53-inhibited FLS could significantly increase IL-17 and IFN-γ expres-sions. It also upregulated Foxp3 expression though had no effect on CD4+CD25high T lymphocytes. Conclusion p53 expression in FLS regulates Th1 and Th17 cells of RA patients, and therefore participate in the pathogenesis of RA.