Expression of angiotensin converting enzyme 2/angiotensin converting enzyme mRNA and protein in myocardium and nephors in spontaneously hypertensive rats
10.3760/cma.j.issn.1671-0282.2009.09.007
- VernacularTitle:自发性高血压大鼠心脏和肾脏血管紧张素转换酶2和血管紧张素转换酶的表达
- Author:
Yinan WU
;
Zhiming GE
;
Fan LI
;
Hong HE
;
Hong JIANG
;
Yun ZHANG
- Publication Type:Journal Article
- Keywords:
Angiotensin converting enzyme 2;
Angiotensin converting enzyme;
Rat;
Heart;
Kidney;
Hypertension
- From:
Chinese Journal of Emergency Medicine
2009;18(9):924-928
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the expressions of angiotensin converting enzyme 2(ACE2)/ACE mRNA in myocardium-nephors and the blood pressure in spontaneously hypertensive rats (SHR).Method A total of 18 male of 12 weeks old SHRs and 18 male of 12 weeks old Wistar Kyoto(WKY)rats were got for the experimental study.Nine SHRs and 9 WKY rats were randomly taken out as controls and sacrificed for detecting the mRNA expressions and protein of ACE2 and ACE.Other halves of rats of both groups were fed for 12 weeks and then sacrificed for detecting of the same biomarkers as in controls.The mRNA expressions of ACE2 and ACE were determined by real-time quantitative RT-PCR.The protein level of ACE2 and ACE were determined by immunohistochemistry.Results Systolic blood pressure(SBP)in SHRs of 12 and 24 weeks old increased compared with that in WKY(all P＜0.01).SBP in SHRs of 24 weeks old increased compared with that in SHRs of 12 weeks old(P＜0.01).The cardiac and renal expressions of ACE2 mRNA in SHRs of 12 and 24 weeks old were all significantly lower than those in WKY rats(P＜0.01).The expressions of ACE mRNA in SHRs of 12 and 24 weeks old were all significantly higher than those in WKY rats(P＜0.05,P＜0.01).The cardiac and renal expression of ACE2 mRNA in SHRs of 24 weeks old was significantly lower than that in SHRs of 12 weeks(P＜0.01).The myocardial and renal expression of ACE mRNA in SHRs of 24 weeks old was significantly higher than that in 12 SHRs of weeks old(P＜0.01).The positive reactions of ACE2 protein found in kidney displayed in brown colur.The ACE2 proetin appeared in uniform distribution of brown granules in WKY rats and comparatively scanty distribution of brown granule in SHRs.Conclusions There are correaltions between the expressions of ACE2/ACE mKNA as well as protein and blood pressure.
