Effects of zoledronic acid on the expression of HIF-1α and VEGF in the osteosarcoma cell line LM8 under hypoxic condition
- VernacularTitle:唑来膦酸对乏氧骨肉瘤细胞HIF-1α和VEGF表达的影响
- Author:
Xianfeng HE
;
Yong HU
;
Penghan YE
;
Yongping RUAN
;
Lei HUANG
;
Feng ZHANG
;
Rongmin XU
- Publication Type:Journal Article
- Keywords:
Diphosphonates/PD;
Osteosarcoma/DT/ME;
Cell hypoxia/DE;
DNA-binding proteins/ME;
Vascular endothelial growth factors/ME
- From:
Journal of Chinese Physician
2009;11(9):1161-1165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of zoledronic acid on the expression of HIF-1α and VEGF in osteosarcoma LM8 cell line under hypoxic condition. Methods The hypoxic culture model was established. After LM8 cells were treated with zoledronic acid, semi-quantitative PCR was used to assess the expression of HIF-1α and VEGF mRNA. The expression of HIF-lct and VEGF protein was de-tected by immunohistochemical staining and ELISA respectively. Results Compared with cells in normoxic conditions, cells in the hypoxic environment and cells treated with zoledronic acid in the hypoxic condition did not show a significant change in the mRNA level of HIF-1α(P >0. 05). However, the protein expression of HIF-1α was markedly decreased in the cells treated with zoledronic acid in the hypoxic envi-ronment. In contrast, both mRNA and protein expression levels of VEGF were down-regulated in the zoledronic acid treatment hypoxic group (P <0.05). Conclusion Under hypoxic conditions in vitro, zoledronic acid inhibited the expression of HIF-1α protein, which decreased VEGF mRNA level and protein expression in osteosarcorna LM8 cell line.
