Effects of inactivation of vitamin D receptor in intestinal tumor growth of APCmin/+ mice
- VernacularTitle:APCmin/+小鼠缺失维生素D受体对肠道肿瘤生长的影响
- Author:
Hong KANG
;
Wei ZHENG
;
Hong YU
- Publication Type:Journal Article
- Keywords:
Intestinal neoplasms/GE;
Receptors;
calcitrioL/BI;
Genes;
bcl-2/BI;
Intermediate filament proteins/BI;
Proto-onco-gene proteins/Bl
- From:
Journal of Chinese Physician
2009;11(9):1174-1177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore effects and mechanism of inactivation of vitamin D receptor (VDR) in intestinal tumor growth of APCmin/+ mice. Methods To generate APCmin/+VDR -/- mice through breeding, the number and size of small intestinal and colonic tumors were assessed and compared between APCmin/+ and APCmin/+ VDR -/- mice. The intestinal tumors were diagnosed with HE stain. The expressions of BCL-2,vimentin-1,Stat-1 and MSH-2 proteins of tumors were determined by immanohistocbemistry and compared be-tween APCmin/+ and APCmin/+VDR -/- mice. Results A comparison between APCmin/+ and APCmin/+ VDR -/- mice revealed that the number of the tumors, which were larger than 3mm, was significantly increased in APCmin/+ VDR -/- mice at 4 months of age (P< 0.01). HE staining indicated fistulous adenomas from small intestine and colon of two groups, Immunostaining showed Stat - 1 level in APC-min/+ tumors were increased and MSH-2 and vimentin-1 levels in APCmin/+ VDR-/- mice were increased, compared to APCmin/+tumors. Conclusion These observations suggested that inactivation of VDR promoted the intestinal tumor growth of APCmin/+ mice.
