Ultrasound mediated microbubble destruction enhances antisense c-myc expression in liver cancer cells SMMC-7721 in vitro
10.3760/cma.j.issn.1004-4477.2009.09.027
- VernacularTitle:超声介导靶向微泡造影剂促进c-myc反义基因在人肝癌细胞的表达
- Author:
Hui JING
;
Wenjia GUO
;
Wen CHENG
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Transfection;
Cell line;
tumor;
Genes;
myc
- From:
Chinese Journal of Ultrasonography
2009;18(9):809-812
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a novel approach of transfecting the exogenous gene safely and effectively mediated by receptor according to the cavitation effect of ultrasound-mediated microbubble destruction. Methods C-myc antisense oligonucleotide(ASODN) and galactosylation polylysine(G-PLL) which was fluoresce in-labeled specificity ligand and SonoVue microbbule were coupled by electrostatic interaction. SMMC-7721 cell with c-myc ASODN were transfected in vitro by ultrasound-mediated microbubble destruction to investigate the effect of c-myc ASODN on it. The c-myc mRNA gene expression of liver cancer SMMC-7721 cells was detected. Results Cultured with 10% SonoVue microbubble and under ultrasound at 1.5 MHz,1.0 W/cm2, 10% duty cycle,60 s respectively,the SMMC-7721 cells had not been changed significantly. Reverse transcription polymerase chain reaction(RT-PCR) assay showed that c-myc expression was decreased in transfected cells(P<0.01). Conclusions Receptor-mediated targeted microbubble destruction by ultrasound can enhance the exogenous gene transfection and expression. It is a promising method in gene therapy of liver cancer.
