Construction of targeted ultrasound contrast agent with anti-IgG monoclone antibody and the evaluation on the effectiveness of its targeted adhesion in vitro
10.3760/cma.j.issn.1004-4477.2009.09.028
- VernacularTitle:携IgG单抗靶向超声造影剂的构建及其靶向效能的体外评价
- Author:
Junhua LI
;
Juefei WU
;
Li YANG
;
Jian LIU
;
Daowen ZHENG
;
Lijing JI
;
Yi YAN
;
Jianping BIN
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Targeted gene repair;
Immunoglobulin G
- From:Chinese Journal of Ultrasonography
2009;18(9):813-816
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct targeted ultrasound contrast agent carried goat anti-mouse IgG antibody (UCA-IgG) and evaluate the effectiveness of its targeted adhesion using parallel plate flow chamber. Methods The ultrasound contrast agent targeted to mouse IgG was designed by conjugating monoclonal antibodies against mouse lgG to the lipid monolayer shell of the agent using biotin-streptavidin. The binding of IgG antibodies to the ultrasound contrast agent were identified by fluorescence in vitro. The attachment and detachment of UCA-IgG to mouse IgG immobilized on a culture dish were assessed in a parallel-plate flow chamber. While the plate lacked mouse IgG,or blocked with large number of goat anti-mouse IgG were served as two control groups. Results UCA-IgG issued a bright green fluorescence, while the contral lipid ultrasound contrast agent didn't show fluorescence. The number of UCA-IgG bound to mouse IgG of experimental group was greater than two control groups,increased with increasing coverslips surface antibody concentrations (P<0. 05),and there was significant positive correlation between the number of UCA-IgG bound to mouse IgG and time of combination (P<0.05). The adhesion rate of experimental group increased with shear stress before 0. 5×10-5 N/cm2 (P<0.05) and then decreased (P<0. 05). There was limited adherence of control groups to the UCA-IgG. The stess of half-maximal detachment was increased with increasing coverslips surface antibody concentrations (P<0.05). Conclusions UCA-IgG could adhere to mouse IgG in the physical conditions. It may provide strong supports for studying other targeted ultrasound contrast agent preliminary and fatherly in vitro.
