Different ultrasound exposure time and dose of microbubble mediated gene transfection to HepG2 cells: in vitro experimental study
10.3760/cma.j.issn.1004-4477.2009.09.026
- VernacularTitle:不同超声辐照时间和微泡剂量介导HepG2细胞基因转染的实验研究
- Author:
Liangjun HU
;
Kaiyan LI
;
Xiaona LIU
;
Xijun ZHANG
;
Wei DONG
;
Yanjun WANG
;
Rong QIN
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Transfection;
Cell line;
tumor
- From:Chinese Journal of Ultrasonography
2009;18(9):805-808
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship of gene transfection efficiency with different ultrasound exposure time and different dose of microhuhble,and to find the appropriate ultrasound parameters for gene transfection. Methods Plasmid encoding enhanced green fluorescent protein(pEGFP) was chosen as a report gene and HepG2 cells were chosen as research object. The HepG2 cells plus pEGFP and different dose of microbubble were exposed to ultrasound(1 MHz,0.5 W/cm2) with varying time. Twenty-four hours later, the expression of EGFP in the cells was observed by fluorescence microscope,the transfection efficiency was assessed by FACS and the cell viability was observed by trypan blue exclusion. Results The expression of EGFP in all experimental groups was different,and the approving transfection efficiency was got by ultrasound exposed for 20 s when the dose of microbubble was 60 μl. Conclusions With fixed ultrasound frequency and power, different transfection efficiency was got when the exposure time and dose of microbubble were different. The appropriate parameter was 20 s,60 μl, which can supply information for further study.
