The effects of pravastatin on expressions of tumor necrosis factorα, Bax and Bcl-2 in apoE-/- mouse

VernacularTitle:普伐他汀对动脉硬化鼠肿瘤坏死因子a和B淋巴瘤-2及相关X蛋白的影响
Author: Yi AN ; Hanjin LIU
Publication Type:Journal Article
Keywords: Pravastatin; Atherosclerosis; Apoptosis
From: Chinese Journal of Geriatrics 2009;28(9):765-768
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of pravastatin on the serum lipids, the tumor necrosis factor (TNF)α and the expressions of Bax and Bcl-2 in apoE-/- mouse. Methods The atherosclerosis model of apoE-/- mouse was induced by high cholesterol diet;and the suspension of pravastatin was administered intragastrieally. After 8 weeks, the levels of serum TNFα and mRNA of aortic TNFα were detected by enzyme linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR), respectively. The expressions of Bax and Bcl-2 were tested by Western blot analysis and aortic histomorphology changes was observed by Hematoxylin-Eosin (HE) stainning. Results In atherosclerosis model of apoE-/- mouse, the serum lipids, the levels of serum and aortic TNFα[(34.8±22.7) μg/L and (180.1±59.1 )μg/L], the expressions of Bax and Bcl-2 and aortic histomorphology were obviously improved by pravastatin. Conclusions It is suggested that the pravastatin can reduce serum lipid, intervene the initiation step of atherosclerosis and regulate endothelial cells' apoptosis.