The relationship between copy number and microsatellite instability of mitochondrial DNA in colorectal cancer

VernacularTitle:结直肠癌线粒体DNA拷贝数与其微卫星不稳定关系的研究
Author: Ping HUANG ; Yunxin ZHANG ; Ting ZHAO
Publication Type:Journal Article
Keywords: Colorectal neoplasms; Microsatellite instability; DNA; mitochondrial; Copy number; Real-time quantitative PCR
From: Chinese Journal of Internal Medicine 2009;48(10):837-840
CountryChina
Language:Chinese
Abstract: Objective To study the relationship between the abnormality of mitochondrinl DNA (mtDNA) copy number and the clinical parameters and microsatellite instability (MSI) in colorectal cancer. Methods Total DNA was extracted from cancer and pericancer tissue from 50 colorectal cancer (CRC) biopsy samples. Non-ceding region sequencing was done and the copy number of mtDNA was quantitated with real-time PCR in mitochondrial NDI gene. The relationship between clinical indicators, mtMSI and mitochondrial copy number was detected. Results The mean copy number of mtDNA 312±185 in the tumor tissue was significantly lower than that 525±125 of the corresponding non-tumor tissue of these patients (P<0.001). No significant correlation was found between mtDNA copy number and other variables including age, gender, pathological type and clinical stage (P > 0. 05). However, there was a significant correlation between copy number and mtMSI (P<0.001). Conclusion There is a significant reduction of mtDNA in CRC patients, which may be caused by mtMSL