Study of the mechanism of dendritic cells induce FBL-3 cells to differentiate into the monocytes in vitro

VernacularTitle:树突状细胞在体外诱导FBL-3细胞分化为单核细胞的研究
Author: Xin YE ; Lianling YU ; Mei ZHAO ; Wengang SONG ; Baoqin ZHOU ; Xue YANG ; Xinglu XU
Publication Type:Journal Article
Keywords: Dendritic cells; Leukemia; Interleukin-12; Manocytes
From: Journal of International Oncology 2009;36(9):712-716
CountryChina
Language:Chinese
Abstract: Objective To investigate mechanism involved in the differentiation of FBL-3 cells(eryth-roleukemia cells)induced by dendritic cells(DC). Methods To cultural the FBL-3 cells together with the DC supematant of different concentration for 72 hours,then we used the Wright's staining methed to record the ma-ture monocyte cell population, the transmission electron microscope to observe the ultramicrostructure, the flow eytoraetry to detect the expression rate of the surface molecular CD14. Results The Wright's staining methed, the transmission electron microscope and the flow cytometry all presented that after being induced by the super-natant of the DC ,the FBL-3 cells can partly differentiate into monocytes,inversion monocyte consistent with the personal characteristics. And the intensity of the DC supernatant was connected with interleukin-12. Conclusion The DC supernatant can induce the FBL-3 cells into monocytoid cell, the differentiated cells correspond to monocyte in macro-appearance,uhramicrostructure and phenotype. The competence of the DC supematant are partly concerned with interleukin-12.