The expression of estrogen sulfotransferase and steroid sulfatase in formalin-fixed paraffin-embedded endometrioid adenocarcinoma samples

VernacularTitle:石蜡包埋子宫内膜样腺癌中雌激素硫酸转移酶和甾体硫酸酯酶基因的表达
Author: Zhanhong JIANG ; Yiyong WU ; Yi ZHANG ; Fenghua WEI
Publication Type:Journal Article
Keywords: Carcinoma; endometrioid; Estrogens; Sulfatases
From: Chinese Journal of Geriatrics 2009;28(10):840-842
CountryChina
Language:Chinese
Abstract: Objective To explore the expression of estrogen sulfotransferase(EST) and steroid sulfatase (STS) in formalin-fixed paraffin-embedded endometrioid adenocarcinoma samples. Methods The RNA of EST and STS in 30 formalin-fixed paraffin-embedded endometrium samples were extracted using Roche products. Results The RNA expression of EST and STS were 0.25±0.03 and 0.08±0.02 respectively and the STS/EST was 0.11±0.08 in normal endometrium. While in endometrioid adenocarcinoma the RNA expression of EST and STS were 0.06±0.02 and 0.24±0.92 respectively and the STS/EST was 4.40±0.64. There were significant differences between these two groups. Conclusions (1) Formalin-fixed paraffin-embedded tissues could be used to study the endometrioid adenocarcinoma. (2) EST is decreased, STS and STS/EST are increased in human endometrioid adenocarcinoma. STS/EST may be related with the prognosis of the endometrioid adenocarcinoma.