Growth pattern and phenotype identification of hepatic stem cells from mice
10.3760/cma.j.issn.1007-8118.2009.10.016
- VernacularTitle:小鼠胎肝干细胞的生长和分化模式及鉴定
- Author:
Weihui LIU
;
Ren LI
;
Kefeng DOU
- Publication Type:Journal Article
- Keywords:
Hepatic stem cell;
Proliferation;
Differentiation;
Identification
- From:
Chinese Journal of Hepatobiliary Surgery
2009;15(10):768-771
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the proliferation and differentiation patterns of hepatic stem cells from mice cultured in vitro isolated identify their characteristics.Methods Mice were divided into 5 groups according to the pregnant (embryo days,ED13,ED15 and ED18) or born age (day0 and 3).Hepatic stem cells were isolated and cultured in vitro.The amount of the stem cells as well as their growing situation and differentiation pattern were observed and compared among different groups and markers of stem cells (CD117,CD90.1,CD49f,c-Met),hepatic cells(AFP) were used to identify the cultures.Resulls The cells with best situation of growing as colonies were obtained from ED15 group.Their expression of specific markers suggested that they were hepatic stem cells.The stem cells isolated from ED15 mice in subculturing proliferated in line pattern and differentiated in reverse line pattern.The expression of AFP varied as normal distribution as cell differentiation development.Conclusion Most cells have characteristics of hepatic stem cells isolated from fetal liver and the number of these cells decreases gradually as embryo duration prolongs.The hepatic stem cells proliferate in line pattern and differentiate into hepatic cells after in vitro culture.
