Effects of SAHA on the differentiation of HCC SMMC-7721 cells
10.3760/cma.j.issn.1007-631X.2009.10.008
- VernacularTitle:HDAC抑制剂SAHA对人肝癌细胞分化诱导作用的研究
- Author:
Shan GAO
;
Daoxiong LEI
;
Zhongli AI
;
Yun WEI
- Publication Type:Journal Article
- Keywords:
liver neoplasms;
Cell differentiation;
Cell cycle;
Gene expression regulation,enzymologic
- From:
Chinese Journal of General Surgery
2009;24(10):802-805
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of SAHA on the differentiation of human hepatocellular carcinoma(HCC)SMMC-7721 cells.Methods Cell morphology was examined by light microscopy;Cell viability was determined by MTT assay;The expression of Alpha-fetoprotein(AFP)and proliferating cell nuclear antigen(PCNA)was analyzed with immunocytochemistry;Flow cytometry(FCM)was used to investigate the cell cycle;The expression of p21 WAF1 mRNA was detected by semi-quantitative RT-PCR.Results Light microscopy showed that SMMC-7721 cells induced by SAHA underwent restorational alterations in morphology which were different from those of nontreated cells but were similar to those of normal cells;MTT assay showed that SAHA inhibited the proliferation of SMMC-7721 cells in a dose-dependent and time-dependent way;Immunocytochemistry assay showed that the expression of AFP and PCNA decreased significantly;FCM analysis showed SAHA could arrest SMMC-7721 cells at G0/G1 phase,with an accumulation of the cells at G0/G1 phase while a decrease of cells at S phase;Semi-quantitative RT-PCR detection revealed that the expression of p21 WAF1mRNA was upregulated remarkably in the cells treated with SAHA.Conclusion SAHA could induce differentiation of human HCC SMMC-7721 cells inhibiting enzymatic activity of HDAC,upregulating the expression of p21 WAFlmRNA as well as causing arrest of HCC cells at G0/G1 phase.
