Screening for pancreatic caner by peptide nucleic acid-mediated one-step K-ras mutation detection assay

VernacularTitle:外周血K-ras基因突变检测在胰腺癌诊断中的应用
Author: Han LIN ; Zhaoshen LI ; Jun GAO ; Yanfang GONG ; Jing JIN
Publication Type:Journal Article
Keywords: Pancreatic neoplasms; Peptide nucleic acids; K-ras gene; Blood circulation
From: Chinese Journal of Pancreatology 2009;9(5):312-314
CountryChina
Language:Chinese
Abstract: Objective To investigate the diagnostic value of determination of the genotypes in codon 12 and 13 of K-ras oncogene in blood samples of patients with pancreatic carcinoma(PC).Methods Blood samples were obtained from 54 patients with pathologically confirmed PC,and 33 healthy controls.The DNAs were obtained in these samples.and then genotype of K-ras mutation was detected by using the PNA-clamping real-time quantitative PCR.Then the correlation between the K-ras genotypes of blood DNA and the clinical characteristics was analyzed.Results K-ras mutations were found in 74.1%(40/54)of patients with PC.There was no such mutation in control samples.The mutations of K-ras was associated with age,lymph node and vessel invasion.poorly differentiated tumor,CA19-9,while it was not associated with sex,tumor location,size of tumor,clinical staging and pathological type.Conclusions The one-step method was highly sensitive for detecting K-ras mutation in blood samples.Detection of circular blood cells harboring K-ras mutation suggested the tumor was highly invasive with poor prognosis.