PI_3 K/Akt signal effects Ephrin-A1 mediated malignancy of HCC cells
10.3760/cma.j.issn.1007-631X.2009.10.004
- VernacularTitle:抑制PI_3 K/Akt信号通路对Ephrin-A1介导的肝癌细胞运动和侵袭能力的影响
- Author:
Gang CHEN
;
Yi WANG
;
Mengtao ZHOU
;
Hongqi SHI
;
Zhengping YU
;
Yefan ZHU
;
Fuxiang YU
;
Yunfeng SHAN
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
Signal transduction;
Ephrin-A1;
PI_3K
- From:
Chinese Journal of General Surgery
2009;24(10):788-791
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of PI_3 K/Akt signal pathway in Ephrin-Al gene mediated invasion,metastasis of Huh-7 cells.Methods Western blot was used to test the protein expression of phosphatidylinositol 3-kinase(PI_3 K)and mitogen-activated protein kinase(MAPK)after Huh-7 cells were treated with Ephrin-A1/Fc fusion protein.According to the protein expression,LY294002 was used to block PI_3 K/Akt pathway specifically,then p-Akt protein expression,mobility and invasive ability of Huh-7 cells were examined.Results In Huh-7 cells actived by Ephrin-Al/Fc fusion protein,p-Akt expression was higher than that in control group(t=4.564,P＜0.05),but there was no difference of p-p38MAPK expression between Ephrin-Al/Fc fusion protein group and IgG/Fc fusion protein group(P＞0.05).PI_3 K/Akt pathway was specifically blocked by LY294002,the p-Akt protein expression decreased in Huh-7 cells,and the mobility and invasive ability mediated by Ephrin-Al in Huh-7 cells decreased(P＜0.05).Conclusions PI_3 K/Akt pathway effects an important role in mobility and invasive ability of Huh-7 cells mediated by Ephrin-A1.
