Vibrio vulnificus: cultivation, identification and antimicrobial susceptibility
10.3760/cma.j.issn.1674-2397.2009.05.009
- VernacularTitle:创伤弧菌的培养鉴定及其对抗菌药物的敏感性
- Author:
Zhigang WANG
;
Pingyang SHAO
;
Xiaoyan WU
;
Kanxiang NI
;
Hongxia LU
- Publication Type:Journal Article
- Keywords:
Vibrio vulnificus;
Cultivation;
Identification;
Antibacterial susceptibility tests
- From:
Chinese Journal of Clinical Infectious Diseases
2009;2(5):293-296
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cultivation, biochemical features and drug susceptibilities of Vibrio vulnificus. Methods Three strains of Vibrio vulnificus were isolated from fatal patients in the Second Hospital of Jiaxing, Zhejiang province. Cultivation, identification and antibacterial susceptibility test were performed. Results Vibrio vulnificus grew on blood agar as dull-gray, opaque colonies with β-hemolysis. The organism presented positive in lactose, cellobiose fermentation and O/129 (10 μg) tests, but lack of inositol and rhamnus. The antibacterial susceptibility tests showed that Vibrio vulnificus strains were sensitive to ciprofloxacin, levofloxacin, imipenem, cefepime, ceftazidime, ceftriaxone, compound sulfamethoxazole and nitrofurantoin, however, resistant to gentamicin, tobramycin, aztreonam and cefazolin. Conclusions Vibrio vulnificus can be isolated from blood, bubbles fluid, and stool. Rapid identification, early diagnosis, and prompt empirical antibacterial therapy are important for reducing the mortality.
