Effect of clozapine exposed in the pregnant day on the development and the expression of MAPK44/42 in brain of filial rats
10.3760/cma.j.issn.1674-6554.2009.10.017
- VernacularTitle:孕期氯氮平暴露对子代大鼠生长发育和脑MAPK44/42表达的影响
- Author:
Haiyan ZENG
;
Guangyu MA
;
Fu CHEN
- Publication Type:Journal Article
- Keywords:
Clozapine;
Filial rats;
Development;
Open field;
Hippocampus;
MAPK44/42;
Pho-MAPK4-4/42
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2009;18(10):905-908
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up the experimental animal model of the filial rats exposed clozapine in the pregnant clay and expect to know what is the effect on the offspring exposed the antipsychiatrie in gestation.Methods Female Wister rats were divided into both the control group (n=17)and elozapine group (n=2.5) which were intraperitoneally injected saline and clozapine respectively from pregnant day 6 to day 15,and their pup rats were examined development indexes such as the physical growth ,neural reflex,neural behavior and the expression of MAPK44/42 in brain in neonatal stage.Results it was shown that clozapine- exposed in pregnant day lead to the retardation of the weight of filial rats in earlier age such as postnatal day 4 (PND4) and PND7(F=12.56/7.51,P=0.001/0.008).As for neural reflex,the time of cliff avoidance of rats was significantly delayed in PND 3 and day 9 (F=4.969/4.348 ; P=0.03/0.041),and the ratio of positive response of air righting decreased compared with the control group in PND13 and PND15 (F=7.959/6.475,P=0.007/0.016).There was no difference between two groups on the behavior of the filial rats in the open field of filial rats.The expression of PhoMAPK44/42 in hippocampi of exposed-clozapine offspring in PND15 was significantly lower than the control' s(F=18.729/23.824,P=0.001) ,however,no difference was demonstrated on the expression of MAPK44/42 in hippocamaias well as the expression of both Pho-MAPK44/42 and MAPK44/42 in forehead cortex in two groups.Conclusion The resuits suggest the aberration of both physical and mental development of filial rats exposed clozapine in gestation.
