Smac/DIABLO and cytochrome c mRNA levels in liver tissue of rats with acute hepatic failure treated by microencapsulated hepatocytes
10.3760/cma.j.issn.1674-2397.2009.06.006
- VernacularTitle:微囊化肝细胞移植对急性肝衰竭大鼠肝组织Smac及细胞色素c mRNA表达的影响
- Author:
Hongjiao WANG
;
Zhiwei XU
;
Junya CHEN
;
Hailong LIN
;
Yiping CHEN
;
Yongping CHEN
- Publication Type:Journal Article
- Keywords:
Cytochromes c;
Second mitochondria-derived activator of caspase;
Hepatocyte transplantation;
Microencapsulated hepatocyte
- From:
Chinese Journal of Clinical Infectious Diseases
2009;02(6):341-344
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate Smac/DIABLO and cytochrome c(cyt-c)mRNA levels in liver tissue of rats with acute hepatic failure treated by microencapsulated hepatocyte.Methods Acute hepatic failure were induced by intraperitoneal injection of D-galactosamine in rats.and the rats were treated with microencapsulated hepatocytes,free hepatocytes and physical saline(contr01),respectively.Smac/DIABLO and cyt-c mRNA in liver tissue was detected by RT-PCR and the mRNA expression levels among three groups were compared.Results Smac/DIABLO and cyt-c mRNA levels in liver tissues of rats with acute hepatic failure were higher than those of normal rata(F=4.345,14.821,47.565,42.178 and 62.961,P<0.05).The peak values of Smac/DIABLO and cyt-C mRNA expressions in free hepatocytes and control groups were at 48 h.while that in microencapsulated hepatoeytes group was at 24 h.Conclusion Smac/DIABLO and cyt-c mRNA expression is an indicator of apoptosis of hepatocytes.
