Effect of Wnt-3a signaling on aggregative growth of dermal papilla cells:a pilot study
10.3760/cma.j.issn.0412-4030.2009.11.014
- VernacularTitle:Wnt-3a信号途径对毛乳头细胞聚集性生长作用的初步研究
- Author:
Yadong YANG
;
Jinjin WU
;
Yuanchao LI
;
Tangyou ZHU
;
Ling CHEN
- Publication Type:Journal Article
- Keywords:
Wnt proteins;
Hair follicles;
Aggregative growth;
Beta-catenin;
Versicans
- From:
Chinese Journal of Dermatology
2009;42(11):767-770
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of Wnt-3a signaling on aggregative growth of dermal papilla cells.Methods Recombinant adenovirus pAdEasy-1/Wnt-3a was constructed at first,and used to transfect cultured dermal papilla cells(DPCs).Then,the growth pattem of DPCs was observed by inverse microscopy;laser confocal microscopy and Western blot were utilized to detect the expressions of Wnt-3a,β-catenin and versican in low-passage DPCs,high-passage DPCs and transfected high-passage DPCs.Results The over-expression of Wnt-3a protein could effectively induce the aggregative growth of DPCs.Laser confocal microscopy showed that the fluorescence intensity of β-catenin and versican increased from 34.16±9.62 and 18.81±9.59 in high-passage DPCs to 87.35±17.28 and 92.18±18.39 in transfected high-passage DPCs(t=11.98,17.88,both P＜0.0 1),respectively.Also,Western blot proved a significant increase of expression intensity of β-catenin(15.27±2.17 vs 60.09±7.24,t=10.10,P＜0.01)and versican(19.32±2.46 vs 58.46±2.78,t=20.63,P＜0.01)in transfected high-passage DPCs compared with untransfected high-passage DPCS.ConclusionsWnt-3a signaling plays an important role in modulating aggregative growth of DPCs,which is mainly through the classical pathway with β-catenin as the key protein.As an important downstream gene of Wnt signaling,vesican is an indispensable part for the modulation of aggregative growth of DPCs.
