Study on the ORF75 subtypes of human herpesvirus-8 in patients with Kaposi's sarcoma
10.3760/cma.j.issn.0412-4030.2009.11.006
- VernacularTitle:人疱疹病毒8型ORF75基因亚型与Kaposi肉瘤的相关性研究
- Author:
Chunlei MA
;
Xiongming PU
;
Weidong WU
;
Dezhi ZHANG
;
Xiujuan WU
- Publication Type:Journal Article
- Keywords:
Sarcoma;
Kaposi;
Herpesvirus 8;
human;
Open reading frames;
Genotype
- From:
Chinese Journal of Dermatology
2009;42(11):748-750
- CountryChina
- Language:Chinese
-
Abstract:
Objective To profile the subtypes of open reading frame 75(ORF75)of human herpesvirus 8(HHV-8)in patients with Kaposi's sarcoma,and to evaluate their relationship with clinical phenotypes and invasiveness of Kaposi's sarcoma.Methods Twenty-five paraffin-embeded tissue specimens of Kaposi's sarcoma were collected in the Department of Dermatology.People's Hospiml of Xinjiang Uygur Autonomous Region.DNA was extracted from these specimens.and nested-PCR was performed to amplify HHV-8 DNA followed bv bi-directional sequencing.Phylogenetic analysis was carried out by using the software DNASTAR,Clustal W program and PHYLIP package so as to identify the ORF75 subtyoe of HHV-8.Results HHV-8 DNA was detected in 21(84%)out of the 25 samples,and 7 cases of AIDS-associated Kaposi's sarcoma were all positive for HHV-8.Among the 21 patients carrying HHV-8 DNA,18 were positive for subtype A ORF75.3 for subtype C ORF75.The ORF75 subtypes had no significant correlation with the presence of mucosal lesions or clinical phenotypes of Kaposi's sarcoma.Conclusions The majority of patients with Kaposi's sarcoma in Xinjiang are infected with HHV-8 of ORF 75 subtype A and C.The ORF75 subtypes of HHV-8 have no correlation with the presence of mucosal lesions or clinical phenotypes of Kaposi's sarcoma.
