A proteomic study of peripheral blood mononuclear cells in systemic lupus erythematosus
10.3760/cma.j.issn.1007-7480.2009.11.014
- VernacularTitle:系统性红斑狼疮患者外周血单个核细胞蛋白质组学研究
- Author:
Chengxiao HU
;
Yong DAI
;
Jianjun LIU
;
Jianfan HE
;
Tianyu LV
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus;
systemic;
Electrophoresis;
gel;
two-dimensional;
Proteomics
- From:
Chinese Journal of Rheumatology
2009;13(11):779-782
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the changes in the protein expression profile of peripheral blood mononuclear cells in systemic lupus erythematosus patients. Methods Peripheral blood was obtained from SLE patients and healthy controls, then mononuclear cells were isolated and the total protein was extracted by one-step method. Two-dimensional gel electrophoresis was performed and then stained with silver. Protein maps were analyzed and differentially expressed protein spots were detected using ImageMaster 2D Platinum 5.0 software. Results Match rates of (71±4)% and (72±4)% was obtained from gels from controls and pati-ents respectively. 791±17 spots were detected from control gels and 781±17 from patient gels. Eleven protein spots were up-regulated and 9 were down-regulated in SLE patients. Five proteins were identified by MS analysis, some of which had previously been shown to play a potential role in the pathogenesis of SLE. Conclusion There are significant changes in the protein expression of peripheral blood mononuclear cells in systemic lupus erythematosus patients. This study could be used as a preliminary work for better understanding of the pathogenesis and immune regulation pathways of SLE from an integrated lymphocyte protein profile perspective.
