Effect of adenoviral-mediated interleukin-10 gene transfection on systemic inflammatory responses in a rat model of ventilator-induced lung injury
10.3760/cma.j.issn.0254-1416.2009.10.017
- VernacularTitle:腺病毒介导的白细胞介素10基因转染对呼吸机相关性肺损伤大鼠全身炎性反应的影响
- Author:
Wuhua MA
;
Si CHEN
- Publication Type:Journal Article
- Keywords:
Adenoviridae;
lnterleukin-10;
Transfection;
Respiration;
artificial;
Respiratory distress syndrome;
adult;
Systemic inflammatory response syndrome
- From:
Chinese Journal of Anesthesiology
2009;29(10):932-934
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of adenoviral-mediated interleukin-10 (IL-10) gene transfection on systemic inflammatory responses in a rat model of ventilator-induced lung injury. Methods Thirty adult male SD rats weighing 200-220 g were randomly divided into 5 groups (n=6 each): group Ⅰ control (group C); group Ⅱ adenovirus without IL-IO gene + 2 h mechanical ventilation (MV) (group B, ); group II1 adenoviruswithout IL-IO gene + 4 h MV (group B_2); group Ⅳ adenovirus with IL-10 gene + 2 h MV (group A_1) and group Ⅴ adenovirus with IL-10 gene + 4 h MV (group A_2). In group Ⅱ-Ⅴ the animals were mechanically ventilated (airway pressure = 25 cm H_2 O) and adenovirus was injected into right ventricle at 48 h before MV. Blood samples were taken from abdominal aorta at the end of 2 h or 4 h MV for determination of serum IL-8 and IL-10 concentrations. The animals were then killed. The pulmonary specimens were obtained for examination of ultrastructure with light and electron microscope. Results Mechanical ventilation significantly increased serum IL-8 and IL-IO concentrations in a duration-dependent manner in group Ⅱ-Ⅴ as compared with group C. Trans-pulmonary administration of adenoviraI-mediated interleukin-10 gene significantly decreased serum IL-8 concentration and increased serum IL-10 concentration and reduced ventilator-induced lung injury alter MV in group A_1 and A_2 as compared with group B_1 and B_2. Conclusion Adenoviml-madiated IL-10 gene transfection can attenuate the systemic inflammatory response and reduce ventilator-induced lung injury.
