Effects of evodiamine on invasion and midkine expression of human colon cancer cell
10.3760/cma.j.issn.1673-4203.2009.11.008
- VernacularTitle:吴茱萸碱对大肠癌细胞侵袭和中期因子基因表达的影响
- Author:
Yongjing ZHOU
;
Yu FAN
;
Youli ZHANG
- Publication Type:Journal Article
- Keywords:
colon carcinoma;
evediamine;
midkine
- From:
International Journal of Surgery
2009;36(11):742-744
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects and mechanism of evodiamine on human colon cancer cell. Methods After human colon cancer SW620 cell were treated with different doses of evodiamine, the growth of anchorage independence of cancer cell was studied by colony formation in soft agar, and invasion ability was determined by Boyden chamber,and the level of mRNA and protein of midkine gene was detected by real time RT-PCR and Western blot assay, respectively. Results Ecodiamine could significantly inhibit both invasion ability and anchorage independence growth in dose-dependent manners. The level of mRNA and pro-tein of midkine of cancer cells treated with evodiamine reduced in time-and dose-dependent manners Conclusion Evodiamine could inhibit invasion of colon carcinoma cell through down-regulating of midkine expression.
