The effect of histone deacetylase inhibitors on cell cycle of breast cancer cell line MCF-7
10.3760/cma.j.issn.1008-6315.2009.12.003
- VernacularTitle:组蛋白去乙酰化酶抑制剂对乳腺癌细胞株MCF-7增殖周期的影响
- Author:
Jianyun NIE
;
Xin LIU
;
Congguo JIN
;
Xiaoqun CHEN
;
Jia LI
;
Yunchao HUANG
- Publication Type:Journal Article
- Keywords:
Breast neoplasm;
Histone deacetylase inhibitor;
Cell cycle
- From:
Clinical Medicine of China
2009;25(12):1238-1240
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of histone deacetylase inhibitors on cell cycle of breast cancer cell line MCF-7.Methods The breast cancer cell line MCF-7 was treated by histone deacetylase inhibitors (TSA、SAHA、CS055、MS-275)respectively and observed by MTT assay after 24 hours,48 hours,72 hours,96 hours.The chosen inhibitor was used to treat MCF-7 cell in different concentration.The flow cytometry was used to detect the Sphase cell and Cyclin A2,Cyclin D1.SPSS 10.0 was used to analyze the data.Results Within the four inhibitors,SAHA showed the most powerful effect of depression of cell growth and time-effect relation.The percentage of Sphase cells and level of Cyclin A2 decreased,the level of Cyclin D1 increased.Conclusions SAHA is the most powerful histone deacetylase inhibitors for breast cancer cell MCF-7,the effect of depression of cell growth shows time-effect relation.Cyclin A2 and Cyclin D1 were involed in this regulation of cell cycle.
