Influences of lysozyme on the gene expressions of matrix metalloproteinase(MMP)-1,-12 and lysyl oxidase in cultured human dermal fibroblasts in vitro
10.3760/cma.j.jssn.0412-4030.2010.01.016
- VernacularTitle:溶菌酶对体外培养的人成纤维细胞基质金属蛋白酶-1和12及赖氨酸氧化酶基因表达的影响
- Author:
Liyuan ZHAO
;
Yuanjun LIU
;
Liyun FAN
;
Junyan ZHANG
;
Yuqing QI
;
Quanzhong LIU
- Publication Type:Journal Article
- Keywords:
Fibroblasts;
Muramidase;
Matrix metalloproteinase 1;
Matrix metalloproteinase 12;
Protein-1ysine 6-oxidase
- From:
Chinese Journal of Dermatology
2010;43(1):36-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influences of lysozyme on the mRNA expressions of MMP-1,-12 and lysyl oxidase(LOX)in cultured fibroblasts in vitro.Methods Primarily cultured fibroblasts isolated from human skin were treated with three concentrations(0.1×10~(-8),1×10~(-7)mol/L)of lysozyme followed by another 24-hour cuhure.Subsequently,total RNA was extracted from the fibroblasts and subjected to RT-PCR for the detection of MMP-1,-12 and LOX mRNA.Results There was a significant difference in the mRNA expressions of MMP-1,-12 and LOX among the fibroblasts treated with the three concentrations of lysozyme (F=6.98,4.44,5.24,respectively,all P<0.05).SNK-q test showed that untreated fibroblasts differed signifi-cantly from those treated with lysozyme of 1×10~(-7) mol/L in the mRNA expression of MMP-1 and MMP-12 (P<0.05),and from those treated with iysozyme of 1×10~(-7) mol/L.and 1×10~(-8)mol/L in the mRNA expres-sion of LOX(both P<0.05),whereas no significant difference was ohserved between fibroblasts treated with lysozyme of 1×10~(-8) mol/L and untreated fibrohlasts or those with lysozyme of 1 x 10~(-7)mol/L in the mRNA expression of MMP-1 and MMP-12.or between fibroblasts treated with lysozyme of 1 x 10~(-8)mol/L and those with that of 1×10~(-7) mol/L in the expression of LOX (all P>0.05).Conclusions Lysozyme upregulates the mRNA expression of MMP-1 and MMP-12 but downregulates the mRNA expression of LOX in cultured fibro-blasts in vitro.
