Methylation state of p16 gene promoter in peripheral CIM~+ T cells of patients with systemic lupus erythematosus

VernacularTitle:SLE患者外周血CD4~+ T淋巴细胞DNA中p16基因启动子区甲基化研究
Author: Zhengliang LI ; Yumei LI ; Hong MA ; Wentao GU ; Hui XU ; Liping LIU ; Wei WU ; Xuejing ZHANG
Publication Type:Journal Article
Keywords: Lupus crythematosus,systemic; CD4-positive T-lymphocytes; Genes,p16; DNA methylation
From: Chinese Journal of Dermatology 2010;43(1):22-24
CountryChina
Language:Chinese
Abstract: Objective To detect the methylation status of p16 gene promoter in CD4~+ T cells of patients with systemic lupus erythematosus(SEE),and its significance in the pathogenesis of SLE.Methods The pl 6 gene promoter methylation in peripheral CD4~+ T cells was detected with the Taqman probe-based realtime PCR(Methylight)technology in 28 patients with SLE and 20 healthy human controls.Results The methylation rate of p16 gene promoter in peripheral CD4~+ T cells was higher in patients with SLE than that in the controls(35.7%VS 10%,x~2=4.11,P<0.05).There was no correlation between SLE disease activity index (SLEDAI)and the normalized index of methylation(NIM)of p16 gene promoter(r_s=-0.29,P>0.05).Conclusion The methylation status of p16 gene promoter is aberrant in CD4~+ T,:ells of SLE patients,suggesting that the hypermethylation of p16 gene plays a certain role in the pathogenesis of SLE.