Expression of OX40/OX40L mRNA in experimental allergic neuritis under influence of Rho-kinase inhibitor
10.3760/cma.j.issn.1006-7876.2010.01.008
- VernacularTitle:Rho激酶抑制剂对OX40及其配体mRNA在实验性变态反应性神经炎中表达的影响
- Author:
Ning ZHANG
;
Gang LI
;
Bo XIAO
;
Yunhai LIU
;
Yan CAI
;
Xingang SUN
;
Jinghui LIANG
- Publication Type:Journal Article
- Keywords:
Neuritis;
autoimmune;
experimental;
rho-Associated kinase;
Receptors;
OX40;
OX40 ligand
- From:
Chinese Journal of Neurology
2010;43(1):26-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of mRNA of OX40 and OX40L in the sciatic nerve,spleen,peripheral blood mononuclear cells and lymph nodes of EAN under the influence of Rho-kinase inhibitor.Methods All 54 female Lewis rats were divided into 3 groups:the EAN group,the EAN+ Rho-kinase inhibitor group and the complete Freund's adjuvant(CFA)group.The rats were sacrificed at 9,17 and 26 days after immunized.Ox40 and OX40L mRNA were detected by RT-PCR which came from spleens,sciatic nerves,peripheral blood mononuclear cells and lymphonodes.Results In EAN+ Rho-kinase inhibitor group,the mRNA expression of OX40 were 0.266±0.031,0.298±0.024 and 0.113±0.018 at 9.17 and 26 days in the sciatic nerve,the expression were 0.453±0.030,0.496±0.100 and 0.220±0.016 in the lymph nodes.The mRNA expression of OX40L were 0.247±0.018.0.298±0.026 and 0.165±0.013 in the sciatic nerve,the expression were 0.283±0.027,0.306±0.011 and 0.161±0.012 in the lymph nodes.The mRNA expression of OX40 and OX40L in EAN+Rho-kinase inhibitor group was lower than EAN group at the three time points(t=2.24-4.89,P＜0.05),and the demyelination and inflammation cells infiltrating were ameliorated in spinal nerve.CFA group didn't show any clinical manifestation.Conclusion Rho-kinase inhibitor may ameliorate tlIe development of EAN through inhabiting the OX40 and OX40L activation.
