Improvements in the establishment and evaluation of cerebral ischemia models made by thread occlusion in rats
10.3760/cma.j.issn.1671-0282.2009.12.004
- VernacularTitle:线栓法制大鼠脑缺血模型操作和评价的改良
- Author:
Bin HE
;
Jinsong ZHANG
;
Binxia SHAO
;
Jie CHENG
;
Fei HUANG
;
Hang XIAO
;
Rang GAO
;
Xiang LU
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Rat;
Animal model;
Model establishment;
Model evaluation;
Achievement ratio;
Tissue staining;
Nerve function score
- From:
Chinese Journal of Emergency Medicine
2009;18(12):1248-1251
- CountryChina
- Language:Chinese
-
Abstract:
Objective To modify the methods of operation and establishment of cerebral ischemia rat models made by thread occlusion. Method We randomly divided 120 male SD rats into a common group (n = 50), an improvement group (n = 60) and a sham-operated group (n - 10). Rats in the common and improvement groups were made into models using the common and improvement methods separately. All models were evaluated on the basis of physical sign indices and 2,3,5-triphenyltetrazolium chloride (TTC) staining. The TTC staining results were taken as gold standards. Then, we compared the achievement ratios of the two groups, and computed the sensitivity and specificity of every physical sign index based on these standards. The χ~2 or correction χ~2 test was used to compare the ratios. Results (1) The achievement ratio in the improvement group was significantly higher than that in the common group (71.67% vs. 52.00%, P = 0.034). (2) The sensitivity of evaluation for both common and improvement methods was 98.55%. However, the specificity of evaluation for the improvement method was significantly higher than that for the common method (100.00% vs. 40.00%, P =0.000). Conclusions The establishment achievement ratio and evaluation correctness of models are obviously elevated by modification of the operation and establishment methods.
