Pancreatic cell apoptosis and endocrine alterations in rats with acute obstructive pancreatitis
10.3760/ema.j.issn.1674-1935.2009.06.005
- VernacularTitle:急性梗阻性胰腺炎大鼠胰腺细胞凋亡及其内分泌变化的实验研究
- Author:
Liping YAN
;
Xiaocong KUANG
;
Yong HANG
;
Hong LI
;
Mingyi ZHU
;
Jie CAI
;
Xiaoping LI
- Publication Type:Journal Article
- Keywords:
Pancreafifis;
Endocrine glands;
Apoptosis;
Rats
- From:
Chinese Journal of Pancreatology
2009;9(6):377-379
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the occurrence of apoptosis and its relationship with endocrine hormones altemtions in rats with acute obstructive pancreatitis(AOP). Methods The model of AOP wag establisbed by ligation of pancreaticobiliary duct.8,12 hrs after operation,the serum insulin,glucagons and amvlase were determined;pancreatic tissues were harvested and apoptotic rate wag evaluated by TUNEL and flow cytometry(Annexin V-FITC/PI assay).Results 8 and 12 hrs after AOP induction,serum amylase levels wefe(1198±687)U/L and(1698±1103)U/L respectively;serum insulin levels were(8.1±5.8)ng/ml and (12.7 ±6.9)ng/ml respectively;sertlm glucagon levels were(6.8±4.6)ng/ml and(7.3±2.9)ng/ml respectively;all these parameters were significantly high than(404±222)U/L,(5.6±2.7)ng/ml and(2.6±2.1)ng/ml in the sham operation group(P<0.05).AnnemnV FITC/PI assay confirmed apoptosis occurred both in exocrine acinus cells and endocrine panclreas islet;and the apoptotic rate wag(20.5±11.2)%and (15.5±8.9)%at 8 and 12 hrs after AOP induction,which wag significantly high than(4.2±1.6)%in the sham operation group(P<0.05).Conclusions Cell apoptosis occurred in both acinar and islet in the model of AOP,and this may be the pathophysiological basis of endocnne hormones alterations in the model of AOP.
