The Neuroprotective Effects of Carnosine in Early Stage of Focal Ischemia Rodent Model.
10.3340/jkns.2014.55.3.125
- Author:
Hui Seung PARK
1
;
Kyung Hoon HAN
;
Jeoung A SHIN
;
Joo Hyun PARK
;
Kwan Young SONG
;
Doh Hee KIM
Author Information
1. Department of Neurosurgery, Seoul Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Focal ischemia;
Carnosine;
Early stage;
Neuroprotective effect
- MeSH:
Anoxia;
Antimycin A;
Blotting, Western;
Brain;
Carnosine*;
Cell Survival;
Enzyme-Linked Immunosorbent Assay;
Infarction, Middle Cerebral Artery;
Injections, Intraperitoneal;
Ischemia*;
Neuroblastoma;
Neurons;
Neuroprotective Agents*;
Oxidative Stress;
Reactive Oxygen Species;
Rodentia*;
Stroke;
Superoxide Dismutase
- From:Journal of Korean Neurosurgical Society
2014;55(3):125-130
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: This study was conducted to elucidate neuroprotective effect of carnosine in early stage of stroke. METHODS: Early stage of rodent stroke model and neuroblastoma chemical hypoxia model was established by middle cerebral artery occlusion and antimycin A. Neuroprotective effect of carnosine was investigated with 100, 250, and 500 mg of carnosine treatment. And antioxidant expression was analyzed by enzyme linked immunosorbent assay (ELISA) and western blot in brain and blood. RESULTS: Intraperitoneal injection of 500 mg carnosine induced significant decrease of infarct volume and expansion of penumbra (p<0.05). The expression of superoxide dismutase (SOD) showed significant increase than in saline group in blood and brain (p<0.05). In the analysis of chemical hypoxia, carnosine induced increase of neuronal cell viability and decrease of reactive oxygen species (ROS) production. CONCLUSION: Carnosine has neuroprotective property which was related to antioxidant capacity in early stage of stroke. And, the oxidative stress should be considered one of major factor in early ischemic stroke.
