Characterization of gene expression and adipokine secretion during differentiation of primary cultured human omental preadipocytes
10.3760/cma.j.issn.1000-6699.2010.01.016
- VernacularTitle:原代培养的人网膜前脂肪细胞分化过程中基因表达和脂肪因子分泌的特征
- Author:
Ming LI
;
Jinhua YIN
;
Kui ZHANG
;
Congyuan WU
;
Jieying DENG
- Publication Type:Journal Article
- Keywords:
Omental adipose tissue;
Preadipocyte;
Primary cell culture;
Adipokine
- From:
Chinese Journal of Endocrinology and Metabolism
2010;26(1):56-59
- CountryChina
- Language:Chinese
-
Abstract:
Stromal preadipocytes from human omental adipose tissues were isolated with enzyme-digesting method,and cultured for inducing differentiation.Preadipocyte factor-1 was abundant during preadipoeyte stage,whereas lipoprotein lipase and PPARγ2 mRNA were rapidly up-regulated at early stage of differentiation and CCAAT/enhancer binding protein expression was increased rather late.In addition,resistin expression and secretion was primarily detectable in preadipocytes,leptin was gradually increased during adipogenesis,whereas adiponectin appeared specifically in mature adipocytes.Insulin-like growth factor-Ⅰ secretion was remarkably detected in both preadipocytes and mature adipocytes.
