Changes and function of Bax and Fas in HL-60 cell apoptosis induced by two fungus polysaccharides
10.3760/cma.j.issn.1673-422X.2009.12.023
- VernacularTitle:Bax和Fas在真菌多糖体外诱导HL-60细胞凋亡中的变化与作用
- Author:
Henglan ZHANG
;
Peie WEN
;
Xia REN
;
Xiaobai SUN
;
Enyu SU
;
Guosheng JIANG
- Publication Type:Journal Article
- Keywords:
Fungi;
Polysaccharides;
HL-60 cells;
Apoptosis
- From:
Journal of International Oncology
2009;36(12):946-948
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the proliferation inhibition and apoptosis of HL-60 cell induced by APS-1 and APS-2 isolated from Polyporus sp. M05 and to investigate its mechanism. Methods The proliferation inhibition was detected by living cells count method,and chosed proper concentration.Flow cytometry with propidium iodide staining was used to detect cell apoptosis. Semi-quantitative RT-PCR was used to detect the expression of apoptosis related gene. Results APS-1 and APS-2 could significantly inhibit the proliferation of HL-60 cells on a time and dose dependent manner. Apoptosis ratio increased to 47. 9% and 26. 8% after HL-60 cells were exposed to APS-1 and APS-2 respectively for 48 h,and the differences had statistical significance (P <0.01). After being induced by APS-1,mRNA of Bax,Fas,Caspase-3 was upregulated. And after being induced by APS-2,mRNA of Bax,Caspase-3 was upregulated,while Fas mRNA did not change. Conclusion APS-1 and APS-2 can inhibit the proliferation and induce apoptosis of HL-60 cells. Mechanism of HL-60 cell apoptosis induced by APS-1 is related to both mitochondrial pathway and Fas signaling pathway,while apoptosis induced by APS-2 is only related to mitochondrial pathway.
