The establishment and evaluation of diagnostic accuracy of AllGlo~(TM) probe-based techniques for invasive aspergillosis
10.3760/cma.j.issn.0578-1426.2010.02.016
- VernacularTitle:一种基于AllGlo~(TM)探针的侵袭性曲霉菌病诊断技术的建立与评价
- Author:
Dansen WU
;
Jianzhen SHEN
;
Xiaoqiang ZHOU
;
Songfei SHEN
;
Xuemei WU
- Publication Type:Journal Article
- Keywords:
Aspergillosis;
Fungi;
Nucleic acid amplification techniques;
Diagnosis
- From:
Chinese Journal of Internal Medicine
2010;49(2):142-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate a diagnostic technique based on the AllGlo~(TM) probe for the invasive aspergillosis. Methods With the self-designed AllGlo~(TM) probes and primers and the standards, two standard curves of the real-time PCR based on AllGlo~(TM) probes were established respectively for A. flaws and A. fumigatus,then its specificity, sensitivity and reproducibility were evaluated respectively. Results The findings indicated that the standard curve of A. flavus was Y = - 3. 003X + 36. 825, and A. fumigatus' was Y = - 3. 052X + 38.016, and their interassay coefficient of variation respectively were 15.60% and 12. 94% , suggesting a good reproducibility. The lowest spore concentration they could be detected was 10 CFU/ml, which equated to 100-1000 copies of internal transcribed spacer (ITS)2 genes, suggesting a good sensibility. They didn't have cross-positive reaction with other fungus, human genome and bacteria, which indicated a good specificity. Conclusion The diagnostic technique based on the AllGlo?probe for the invasive aspergillosis possessed a good sensitivity, good specificity and deadly accuracy.
