Study on mechanism of reactive oxygen species in regulating adiponectin expression in adipocytes
10.3760/cma.j.issn.1000-6699.2010.01.015
- VernacularTitle:氧自由基降低脂肪细胞脂联素表达的机制研究
- Author:
Baoying CHEN
;
Jingguo WEI
;
Wei WANG
;
Longxiao WEI
;
Guangbin CUI
;
Jun YU
- Publication Type:Journal Article
- Keywords:
Oxidative stresss;
Adipoeyte;
Adipokines;
Adiponectin
- From:
Chinese Journal of Endocrinology and Metabolism
2010;26(1):52-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the signaling cascades involved in the regulatory effects of reactive oxygen species(ROS)on adiponectin expression in 3T3-L1 adipocytes.Methods 3T3-L1 cells were cultured and differentiated into mature adipocytes.Adiponectin mRNA expression was evaluated by quantitative real-time PCR.The signaling pathways associated with ROS-decreased adiponectin expression were screened by Bioplex phosphoprotein assays and various protein kinase inhibitors.Results As an important ROS,H_2O_2activated several signaling pathways including extracellular signal regulated protein kinase 1/2(ERK1/2),c-Jun N-terminal kinase (JNK),protein kinase B(Akt),p70 S6 kinase(p70 S6K),and Janus kinase/signal transducer and activator of transcription(JAK/STAT).Akt and JAK/STAT inhibitors completely reversed H_2O_2-decreased adiponectin expression(both P<0.01).Conclusions ROS markedly down-regulates adiponeetin expression in 313-L1 adipocytes by activating Akt and JAK/STAT signaling pathways,which may contribute to the development of obesity and its related diseases.
