Effects of endogenous NO on sensitivity to chemotherapy in human breast cancer cell line
10.3760/cma.j.issn.1673-4203.2010.01.011
- VernacularTitle:内源性NO对人乳腺癌细胞化疗敏感性的影响
- Author:
Wei TU
;
Jian WEN
;
Wanqing QIAO
;
Man ZHAO
;
Zuofu YU
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Nitric oxide;
Interleukin-1β;
Chemosensitivity
- From:
International Journal of Surgery
2010;37(1):32-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of endogenous NO on the chemosensitivity of human breast cancer cell. Methods MCF-7 cells were cultured as monolayer, incubated with cytokine IL-1β. The pro-duction of NO was detected by NO assay. The expression of iNOS protein was measured by Western blotting. Establishing control group and experimental group, the chemosensitivity of MCF-7 cells incubated by L-NMMA and L-Arg to ADM and 5-Fu was studied by MTT assay. Results There was a positive correlation of dose-dependence between NO production and IL-1β concentration. MCF-7 cells expressed plenty of iNOS by induetion of IL-1β. There was no significant difference on iNOS whether L-NMMA and L-Arg existed or not. Incubating MCF-7 cells with 0. 5 μmol/L or 1 μmol/L ADM, the survival rate of experiment group was remarkablely decreased(P < 0.05) ; L-NMMA significantly increased survival rate of experiment group(P < O. 05) ; L-Arg decreased survival rate of experiment group(P < 0.05). Conclusion The induction of IL-113 in MCF-7 cells can increase the production of endogenous NO, which increases MCF-7 cells' sensitivity to chemotherapy.
