Study on antibacterial activity of combined antimicrobial agents against imipenem-resistant Acinetobacter baumannii in vitro
10.3760/cma.j.issn.1000-6680.2010.01.004
- VernacularTitle:耐亚胺培南鲍曼不动杆菌的体外联合药物敏感试验
- Author:
Ji ZENG
;
Liang DENG
;
Jie CHEN
;
Yanchun HONG
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Cefoperazone;
Sulbaetam;
Minocycline;
Microbial sensitivity tests
- From:
Chinese Journal of Infectious Diseases
2010;28(1):14-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antibacterial activity of combined antimicrobial agents for imipenem-resistant Acinetobacter baumannii in vitro,so as to provide evidence for guiding clinical practice.Methods Twenty-seven isolates of irnipenem resistant Acinetobacter baumannii were collected from March,2007 to February.2008.The minimal inhibitory concentrations(MIC)of the isolates against eleven frequently used antibiotics were determined by E test and agar dilution.Combined drug sensitivity testing was performed by broth checkerboard titration assay.Synergism between minocycline and cefoperazone-sulbaetam was analyzed by time-killing curve.Results The results from checkerboard titration assay and time-kill studies showed that the synergy rates between minocycline and cefperazone-sulbactam,minocycline and amikacin,minocycline and ciprofloxacin were 74.1%,28.0%and 48.1%,respectively.The synergy rates between amikacin and cefoperazone-sulbactam,anaikacin and ciprofloxaein were 23.1%and 37.0%,respectively.The synergy rate between cefoperazone-sulbactam and ciprofloxacin was 7.1%.With combination therapy.both MICs of cefoperazone-sulbactam and MIC of minocyeline decreased to lower than breakpoints.According to time-killing curve,the synergy rate between cefoperazone-sulbactam and minocycline was 100%.Conclusion Minocycline and cefoperazone-sulbaetam can be used for treating infections caused by multidrug resistant Acinetobacter baumannii.
