A study of mechanism of modulation of NF-κB activity of HepG2 cells by BC047440 genes

VernacularTitle:BC047440基因调控HepG2的NF-κB活性机制的实验研究
Author: Xiaobing HUANG ; Ping LIANG ; Jing LI ; Lu ZHENG ; Shicheng LIU ; Keqiang HAN ; Hongzhi ZHAO ; Yanbang CHI
Publication Type:Journal Article
Keywords: Gene expression regulation; BC047440 gene; RNA interference; DNA microarray
From: Chinese Journal of Hepatobiliary Surgery 2010;16(2):134-137
CountryChina
Language:Chinese
Abstract: Objective To investigate the mechanism that BC047440 gene regulates nuclear fac-tor κB sigal passway and analyze the differential expression gene between HepG2 cells and HepG2 cells BC047440 gene silenced by RNAi using 35K Human Genome Array. Methods The differential expres-sion gene between HepG2 cells and HepG2 cells with BC047440 gene silenced was analyzed by 35K Human Genome Array, and the data were submitted to the database and MAS system of Capitalbio Corporation.Then TRAF6 was confirmed by RT-PCR test. Results Among the total 35000 probe sets, the expression of 59 genes was down-regulated for more than 50% and 130 genes were up-regulated more than 2 fold in the silencing group when compared with normal controls. TRAF6 mRNA was decreased for 29.5% in silicening HepG2 compared with that of wild HepG2 by RT-PCR, which is similar to human genome array(23.06%).Conclusion The high throughput and effective oligomicroarray can analyze the differential expression gene and BC047440 gene might regulate NF-κB signal pathway inderectly by TRAF6.