Effect of losartan on expression of PTHrP and PTH1R induced by high glucose in NRK-52E cells
10.3760/cma.j.issn.1000-6699.2010.02.005
- VernacularTitle:高糖诱导NRK-52E细胞甲状旁腺素相关肽及其受体1表达及氯沙坦干预研究
- Author:
Huasheng LIANG
;
Yuhua ZHONG
- Publication Type:Journal Article
- Keywords:
PTHrP;
PTH1R;
Losartan;
NRK-52E cells;
High glucose
- From:
Chinese Journal of Endocrinology and Metabolism
2010;26(2):111-113
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of losartan on high glucose up-regulated expression of parathyroid hormone-related peptide (PTHrP) and its type 1 receptor (PTH1R) in NRK-52E cells. The expression of PTHrP and PTH1R were detected by RT-PCR and Western blot in control group (0 mmol/L glucose) ,normal glucose group (5 mmol/ L glucose) ,moderately high glucose group (16.7 mmol/L glucose) ,high glucose group (25 mmol/L glucose) ,and after intervention by 10 μmol/L losartan for 72 h (only Western blot). The expression of PTHrP and PTH1R were up-regulated by high glucose (PTHrP mRNA : 0. 66 ± 0.08, 0. 84 ± 0. 13,1.57 ± 0. 15, and 1.73 ± 0.21 ; PTHrP protein :0.63±0.12,0.68±0.06,1.02±0. 11, and 1.04±0.08 ; PTH1R mRNA :0.26±0. 08,0.28±0.07,2.35± 0. 10,and 2.47±0. 05 ; PTH1R protein:0. 88±0. 05,0. 87±0. 10, 1.05±0. 11, and 1.12±0. 09) ,and losartan inhibited the effects of high glucose (PTHrP 0.74±0. 15, PTH1R 0.98±0.06, both P<0.01). The results suggest that losartan could inhibit the expression of FTHrP and PTH1R induced by high glucose in NRK-52E.
