Mutation analysis of ferrochelatase gene in a pedigree with erythropoietic protoporphyria
10.3760/cma.j.issn.0412-4030.2010.02.005
- VernacularTitle:红细胞生成性原卟啉病一家系亚铁螯合酶基因突变检测
- Author:
Junhong MA
;
Shengxiang XIAO
;
Jingang AN
;
Xiaopeng WANG
;
Qingqiang XU
;
Yingying DONG
;
Yiguo FENG
- Publication Type:Journal Article
- Keywords:
Protoporphyria;
erythropoietic;
Ferrochelatase;
Mutation
- From:
Chinese Journal of Dermatology
2010;43(2):85-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize the inheritance of erythropoietic protoporphyria (EPP) by detecting the mutations of ferroehelatase (FECH) gene in a Chinese family with EPP. Methods Peripheral blood samples were obtained from 4 patients and 3 unaffected individuals in a family with EPP, as well as from 50 unrelated healthy human controls. PCR was performed to amplify all the 11 exons and flanking sequence of FECH gene followed by direct sequencing. Results A splicing mutation,I.e., IVS3+1G→A, was identified in the proband as well as his symptomatic sister, cousin, grandfather and asymptomatic mother, but not in his asymptomatic father, grandmother, or unrelated healthy controls. The genotypes IVS1-23 T/C and IVS3-48 C/T were noted in the proband, his father, sister, cousin and grandfather, but absent in his mother or grandmother who carried IVS1-23 C/C and IVS3-48 T/T genotypes. Conclusions A novel splicing mutation is found in the FECH gene in a Chinese EPP family, which, together with two lowly expressed alleles IVS1-23T and IVS3-48C, is likely to be responsible for the clinical phenotype of EPP in this family.
