Effects of RNA interference-mediated adiponectin gene silence on glucose-lipid metabolism in ApoE gene knock-out mice
10.3760/cma.j.issn.1000-6699.2010.02.015
- VernacularTitle:RNAi介导的脂联素基因沉默对ApoE基因敲除小鼠糖脂代谢的影响
- Author:
Rui LIU
;
Ling LI
;
Gangyi YANG
;
Xun SUN
;
Wei ZHU
- Publication Type:Journal Article
- Keywords:
RNAi;
Adiponectin;
Insulin resistance;
Glucose-lipid metabolism
- From:
Chinese Journal of Endocrinology and Metabolism
2010;26(2):143-145
- CountryChina
- Language:Chinese
-
Abstract:
The effect of RNA interference-mediated adiponectin gene silence on glucose-lipid metabolism in apolipoprotein E gene knock-out(ApoE~(-/-)) mice was studied. The plasma adiponectin level in ApoE~(-/-) mice with high-fat diet and adiponectin short hairpin RNA (shRNA) adenovirus injection (ADI group) was significantly lower than that in ApoE~(-/-)mice with high-fat diet(HF group), normal-chow(NF group)and naked adenovirus injection (GF, both P<0.01). Fasting blood glucose, fasting plasma insulin, free fatty acids, total cholesterol, triglycerides, low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), and body weight were significandy elevated in three high-fat diet groups compared with in NF group, (P<0.01). However, fasting plasma insulin, free fatty acids, total cholesterol, triglycerides, LDL-C, and HDL-C in ADI group were significantly higher than those in HF and GF groups(P<0.01 or P<0. 05). A typical clinical phenotype of glucose-lipid metabolism and insulin resistance in ApoE~(-/-) mice can be induced by high-fat feeding and adiponecting gene silencing.
