The protein function of lipoprotein lipase with Asn291Ser and Lys312insC compound mutation
10.3760/cma.j.issn.1000-6699.2010.02.018
- VernacularTitle:Asn291Ser和Lys312insC联合突变脂蛋白脂酶蛋白功能研究
- Author:
Juan WANG
;
Yaomin HU
;
Xuerong LIU
;
Shengxian LI
;
Wei LIU
- Publication Type:Journal Article
- Keywords:
Lipoprotein lipase;
Gene knockout;
Compound mutation
- From:
Chinese Journal of Endocrinology and Metabolism
2010;26(2):150-152
- CountryChina
- Language:Chinese
-
Abstract:
To study the function of lipoprotein lipase (LPL)with Asn291 Ser and Lys312insC compound mutation in LPL gene knockout heterozygous (LPL~(+/-)) mice. The results showed that triglycerides, free fatty acids, blood glucose and weight of LPL~(+/-) mice were higher than those of c57 mice(P<0. 05). The expressions of LPL Mrna and LPL protein of LPL~(+/-) group were lower than those of c57 group(P<0.05). The injection of Asn291Ser +Lys312insC protein caused little change of the lipid mass and LPL activity,but the injection of normal LPL protein induced obvious decrease of lipid mass and increase of the LPL activity.
