Vascular smooth muscle cell senescence induced by reactive oxygen species and intervention effect of dehydroepiandrosterone
10.3760/cma.j.issn.0254-9026.2010.02.020
- VernacularTitle:活性氧诱导血管平滑肌细胞衰老及脱氢表雄酮的干预研究
- Author:
Yunjun RUAN
;
Saizhu WU
;
Jian QIU
;
Fengying DONG
;
Wenyan LAI
- Publication Type:Journal Article
- Keywords:
Reactive oxygen species;
Dehydroepiandrosterone;
Muscle cell,smooth,vascular
- From:
Chinese Journal of Geriatrics
2010;29(2):154-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the onset of vascular smooth muscle cell (VSMC) senescence induced by tert-butyl hydroperoxide (t-BHP) and the intervention effect of dehydroepiandrosterone (DHEA). Methods The VSMCs were divided into four groups: blank control group, t-BHP group (incubated with 80 μmol/L t-BHP for 72 h), 10 nmol/L DHEA intervention group (pretreated with 10 nmol/L DHEA 30 min before t-BHP) and 100 nmol/L DHEA intervention group (pretreated with 100nmol/L DHEA 30 min before t-BHP). Two ageing markers of ageing associated β-galactosidase activity and cell proliferation activity were adopted as main indexes. β-galactosidase activity was measured with immunocytochemical method and cell proliferation activity was measured with flowcytometry. Results After continuous treatment with 80 mmol/L t-BHP for 72 h, the ratios of G0/G1 phase cells and SA-β-galactosidase staining positive cells increased as compared with blank controlgroup [(89.4±3.4)% vs. (49.5±5.5)%, (3.5±1.2)% vs. (75.3±4.3)%], which indicated that VSMCs senescence were successfully induced by t-BHP. While the above changes were smaller in 100 nmol/L DHEA intervention group than in t-BHP group. Conclusions With ageing,accumulation of damage produced by reactive oxygen species may be an important mechanism causing the onset of VSMCs senescence. DHEA may be able to retard the progression of VSMCs senescence through antioxidant effect.
