Establishment of the isolated perfused rat liver model and its modification
10.3760/cma.j.issn.0254-1785.2009.08.006
- VernacularTitle:大鼠肝脏离体再灌注模型的建立及改进
- Author:
Wenyu ZHAO
;
Haiyun XIONG
;
Qing YUAN
;
Youhua ZHU
- Publication Type:Journal Article
- Keywords:
Rats;
Liver transplantation;
Reperfusion;
In vitro
- From:
Chinese Journal of Organ Transplantation
2009;30(8):469-472
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up the isolated perfused rat liver model. Methods Rat livers were harvested after the cannulation of the portal vein and bile duct. The reperfusion solution was Krebs-Henseleit solution containing bovine albumin serum and sodium taurocholate. The original CZ-1 isolated perfused rat liver system contained two subsystems: recirculating perfusion system and heat-exchange system. Then we modified the original CZ-1 system and omitted the heat-exchange system.The modified CZ-1 system consisted of a thermostatically regulated water bath,a peristaltic pump,a 4-neck round-bottom flask, a flow meter, an in-line manometer, a glass organ chamber, an iron support and a set of recirculating pipe line. Then the livers were connected via the portal vein to the modified CZ-1 system for 120 min. After 120 min reperfusion, bile production was evaluated. Routine HE staining and electron microscopic examination of hepatic tissues were also performed. Results The was not significantly different from that reported by references. Hepatic tissues in reperfusion group were also morphologically normal Conclusion The CZ-1 isolated perfused rat liver system was cost-effective and reliable to use. It was easy to run and is the ideal model for investigation of organ preservation solution.
