Relationship between the erythrocyte CR1 genomic density polymorphism and erythrocyte immune function in children with asthma
10.3760/cma.j.issn.1673-4904.2010.03.003
- VernacularTitle:哮喘儿童红细胞补体受体1基因多态性及红细胞免疫功能的研究
- Author:
Jinhua FENG
;
Pu QIN
;
Yuhong JIANG
- Publication Type:Journal Article
- Keywords:
Asthma;
Immunity,cellular;
Receptors,complement;
Gene polymorphism
- From:
Chinese Journal of Postgraduates of Medicine
2010;33(3):7-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the hereditary susceptibility of children with asthma through studying the relationship between erythroeyte CR1 genomic density polymorphism and erythrocyte immune function. Methods The rates of RBC-C3_(3b)RR and RBC-ICRR were detected to the asthma group consisted of 65 children with asthma and the control group consisted of 28 normal children. The CR1 activity and genomic density polymorphism of erythrocyte from the two groups were detected by Hind Ⅲ restriction enzyme digestion, polymerase chain reaction restriction fragment length polymorphism. Results Frequencies of high expression gene (HH), mid expression gene (HL) and low expression gene (LL) genotypes were 43.08% (28/65), 36.92% (24/65) and 20.00% (13/65) in asthma group, and 78.57% (22/28), 17.86% (5/28) and 3.57%(1/28) in control group respectively. A significant difference was found in the distribution frequency of CR1 genotype between the two groups(P< 0.01).The rates of RBC-C_(3b)RR were significant lower and the rates of RBC-ICRR were significant higher in asthma group than those in control group (P < 0.01). The rates of RBC-C_(3b) RR in HH, HL and LL were decreased in order (P < 0.01),while the rates of RBC-ICRR in HH,HL and LL were increased in order (P < 0.01). Conclusion It suggests that CR1 gene polymorphism may play an important role in determining susceptibility to asthma.
