Effects of platelet activating factor and its receptor antagonist on proliferation of hepatic stellate cells

VernacularTitle:血小板活化因子及受体拮抗剂对肝星状细胞增殖的影响
Author: Jun LIU ; Xuemei MA ; Yu WANG ; Zhongtao ZHANG
Publication Type:Journal Article
Keywords: Platelet activating factor; Hepatic stelate cell; Proliferation
From: Chinese Journal of Hepatobiliary Surgery 2010;16(2):122-124
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of platelet activating factor on the proliferation of cultured hepatic stellate cells in vitro. Methods LX-2 cell line was chosen as the study model of the activated hepatic stellate cells. MTT colo- rimetric assay was used to evaluate the proliferation of HSCs. Cell cycle was evaluated by ways of flow cytometry with PI staining and BrdU incorporation.Results OD_(492) value of PAF groups at concentration from (1 × 10~(-8)-1 × 10~(-3) mol/L), were higher than that of control group (P < 0.05). OD_(492) value of PAF (1×10~(-6)) + PAF-RA × 1 (10~(-7)-1 × 10~(-4)mol/L) groups was less than that of PAF (1 ×10~(-6)mol/L) group (P<0.05). S% and PrI of PAF groups increased significantly. Conclusion PAF can increase the proliferation of hepatic stellate cells in a dose-dependent manner while PAF receptor antagonist reduces the proliferation of hepatic stellate cells induced by PAF.