Experimental study on the maintenance of porcine hepatocyte morphology and function by culturing with bone marrow mesenchymal stem cells
10.3760/cma.j.issn.1007-8118.2010.02.017
- VernacularTitle:骨髓间充质干细胞维持猪肝细胞形态与功能的实验研究
- Author:
Jinyang GU
;
Xiaolei SHI
;
Yue ZHANG
;
Yitao DING
- Publication Type:Journal Article
- Keywords:
Liver failure;
Porcine hepatocyte;
Bone marrow mesenchymal stem cell;
Co-culture;
Morphology;
Functionality
- From:
Chinese Journal of Hepatobiliary Surgery
2010;16(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish porcine hepatocyte co-culture system with bone marrow mes-enchymal stem cells (MSCs) in vitro for the ideal cell source of bioartificial liver. Methods Mononu-clear cells were isolated from bone marrow aspirate extracted from the anterior superior lilac spine of swines (n=3) by 1.077 g/ml density gradient centrifugation. MSCs of passage 3 and primary hepato-cytes harvested by a two-step in situ collagenase perfusion technique were randomly distributed, and the morphological and functional changes of heterotypic interactions were characterized. Results The purity of the third passage MSCs and primary hepatoeytes was more than 90 % and 99%, respectively.Hepatocyte viability was greater than 95 %. A rapid attachment and self-organization of three-dimen-sional hepatocyte spheroids were encouraged in co-culture. Heterotypic junctions remained similar to that of hepatocytes in vivo. Hepatocyte performance levels such as albumin secretion and urea synthe-sis were all significantly enhanced in co-culture group compared with hepatocyte homo-culture (P<0.05). The best hepatic function levels were achieved on day 2 and moderately decreased in the following co-culture days. Conclusion Co-cultivation of porcine hepatocytes and MSCs may preserve hepatocyte morphology and function, which could contribute to the functional bioartificial liver.
